Qwen3-4B-Instruct-2507实际应用：自动生成周报系统搭建
PR-619AbMoleM2565是一种广谱的去泛素化酶DUBs抑制剂其作用机制涉及通过增强蛋白泛素化和诱导内质网应激激活IRE
GRP
caspase-
CHOP等来调控细胞应激反应从而抑制细胞增殖并促进凋亡。
PR-619在猪卵母细胞体外成熟实验的研究IVM中显著降低成熟率浓度为10μM和15μM[1]。
PR-619在膀胱尿路上皮癌UC细胞系T24和BFTC-905中与Cisplatin顺铂CDDP联用可增强顺铂的细胞毒性[2]PR-619在食管鳞状细胞癌ESCC细胞中通过激活caspases和PARP裂解诱导G0/G1期细胞周期阻滞[3]。
PR-619能在动物模型中如BALB/c小鼠结直肠癌模型中与抗PD1联用显著抑制肿瘤生长[4]并在小鼠模型中通过上调parkin介导的线粒体自噬表现为LC3-II/LC3-I比值升高和LAMP1水平增加发挥细胞视网膜神经节细胞保护作用[5, 6]。
此外PR-619在Sprague-Dawley大鼠中可减轻肾纤维化抑制Smad4的表达[7]以及在C57BL/6小鼠眼高压模型中通过parkin依赖的线粒体自噬途径保护视网膜神经节细胞RGCs[6]。
研究还显示PR-619在10 mg/kg剂量下对白血病MV
细胞移植瘤小鼠模型表现出显著的抑制活性[8]。
参考文献及鸣谢[1] Wang, Y.; Zhuang, L.; Chen, X.; et al. Inhibition of deubiquitinases alters gamete ubiquitination states and sperm-oocyte binding ability in pigs.Animal reproduction science2017,187, 64-
[2] Kuo, K. L.; Liu, S. H.; Lin, W. C.; et al. The Deubiquitinating Enzyme Inhibitor PR-619 Enhances the Cytotoxicity of Cisplatin via the Suppression of Anti-Apoptotic Bcl-2 Protein: In Vitro and In Vivo Study.Cells2019,8(
.[3] Lin, W. C.; Chiu, Y. L.; Kuo, K. L.; et al. Anti-tumor effects of deubiquitinating enzyme inhibitor PR-619 in human chondrosarcoma through reduced cell proliferation and endoplasmic reticulum stress-related apoptosis.American journal of cancer research2023,13(
, 3055-
[4] Wu, J.; Liu, C.; Wang, T.; et al. Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis.Immunology2023,170(
, 439-
[5] Hu, X.; Zhang, J.; Ma, H.; et al. The broad-spectrum deubiquitinating enzyme inhibitor PR-619 protects retinal ganglion cell and augments parkin-mediated mitophagy in experimental glaucoma.Scientific reports2024,14(
,
[6] Hu, X.; Zhuang, D.; Zhang, R.; et al. The small molecule inhibitor PR-619 protects retinal ganglion cells against glutamate excitotoxicity.Neuroreport2020,31(
, 1134-
[7] Soji, K.; Doi, S.; Nakashima, A.; et al. Deubiquitinase inhibitor PR-619 reduces Smad4 expression and suppresses renal fibrosis in mice with unilateral ureteral obstruction.PloS one2018,13(
, e
[8] Wang, B.; Wu, J.; Wu, Y.; et al. Discovery of 4-(((4-(5-chloro-2-(((1s,4s)-4-((2-methoxyethyl)amino)cyclohexyl)amino)pyridin-4-yl)thiazol-2-yl)amino)methyl)tetrahydro-2H-pyran-4-carbonitrile (JSH-
as a novel highly selective and potent CDK9 kinase inhibitor.European journal of medicinal chemistry2018,158,
6
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